Regarding stranded-specific library preparation by dUTP method, as far as I know, the dUTP is used during the synthesis of the second strand, which will be degraded before PCR amplification step, so just the first strand is sequenced. What about the sequencing of the second strand (coding or sense strand)? I searched on the net a lot, but I didn't find a clear answer for it. Could you please kindly tell me about the second strand sequencing? it will be great if there is any schematic image.