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For cufflinks error of using gff files

Hie everyone, I am using bowtie for the alignment of the reads. The output of the bowtie is used to cufflink.For cufflink I am using -g -GTF option to give the reference genome gff file. But cufflinks dose not take that. I am very new in this field and I have no idea how to solve this. I request you all to give some help to me.

Regards, Bandana

rna-seq rna-seq assembly gene

Please indicate the error that you are getting..

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