staying up to date with seq database
I did some phylogenetic and statistical analysis of several sequences from bacterial genomes 1 yr ago. Now I found that some of the sequences are removed from refseq. So what does it mean? The analysis is invalid? I found many papers with seq ids not in the database anymore. I am wondering how to cope with it. How old seq analysis is valid?
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Can you provide an example of accession number that was removed? Generally there should be some notes (or forward links) when changes like this happen.
As with everything, data analysis you did was valid at that particular point in time (I assume it was published). If the underlying database changed (which you have no control over) there is not much you can do. That change does not invalidate the original analysis but it may not be as useful since it can't be reproduced.