NGS_Where to start
I am interested in sequencing the promoter regions, the exons and the splice junctions of three genes. What is the best way to find the locations of all the exons and generate a bed file using hg19 as the reference? Also, I understand that each gene may generate multiple transcripts, is it best to use the sequence that produces the longest transcript or the RefSeq gene? Finally, what questions should I be asking that I am not?
Thank you all!
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fowler.trentd •
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fowler.trentd •
1 answer
- biomart
- Lacking other information, just do all of them.
- Probably, but since we don't know what your actual goals are (other than doing some targeted sequencing) it's tough to say more. Since you have "ngs" in the tags, I presume you're interested in kits that will help with the targeted capture step of library preparation. I'd ask that on seqanswers rather than here.
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