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how to find the primer for the promoter of VEGF gene?

Hi,

I want to determine the expression level of VEGF-A by ChIP-qPCR, as active h3k4me3 are positively related with VEGF-A expression. the promoter regions of VEGF-A is usually highly methylated at h3k4. So I need to design a primer that covers the promoter regions of VEGF-A. Does anyone know how to do that? Thank you so much!

chip-qpcr vegf primer design
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updated by natasha.sernova natasha.sernova • written by dcheng1 • dcheng1

1 answer

Look at this article:

Isolation and cloning of human VEGF promoter region

http://www.ijsrp.org/research-paper-0812/ijsrp-p0815.pdf

The authors used some primers and published their sequences.

Below you can see a paper from 1991, but the authors were succesful as well, am I right?

The Human Gene for Vascular Endothelial Growth Factor

http://www.jbc.org/content/266/18/11947.full.pdf

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written by natasha.sernova natasha.sernova

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