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Double check plasmid existence from NGS assembly

We assembled single species PacBio data into a chromosome and a plasmid. Is it possible to validate plasmid existence just in silico, not wet lab.

Thanks

bacteria

2 answers

Hi, what about the coverage level, sometimes when plasmids are in multiple copy number we can see that in the coverage rate.

I recently came across this: "plasmidSPAdes: Assembling Plasmids from Whole Genome Sequencing Data", maybe you could give it a try.

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