Fastsimcoal for transcriptome data
Hello,
Is it possible/ good idea to use fastsimcoal to infer demography using transcriptome data ?
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biobudhan •
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Fastsimcoal generates simulated data. Not an expert but I would not be very comfortable with using transcriptome data for demography as accurate SNP calling is a challenge (uneven coverage due to differences in expression, errors in RTPCR, allelic imbalance etc) and exonic regions are under selection.