This is a test version of Biostars. For the public version, visit https://www.biostars.org.
GFF3 to GTF file conversion in RSEM error

I have a GFF3 file I am trying to use for building a reference for RSEM:

I am using the command:

./rsem-prepare-reference --bowtie --gff3 xxx.gff  xxx.fa  xxx

The Error I get is:

Traceback (most recent call last):
 File "/local/bin/rsem-gff3-to-gtf", line 228, in <module>
print("Loaded {} lines".format(line_no))
ValueError: zero length field name in format

Anyone got an idea what's wrong? I am using Python version 3.5 on my sever. I'm assuming it's coming from the python end of the script 'rsem-gff3-to-gtf' script which rsem-prepare-reference calls?

Thanks.

gff3 rsem gtf

I tested a bacterial genome and gff3 file and they work fine. Must be the file I have;HOWEVER, saying that, it works fine with STAR. Any ideas what the error refers to?

If anyone experiences this problem, you can go to enter link description here and use this nice little tool to convert. Must be a problem with latest RSEM release.

0 answers

No answers yet.

Log in to answer this question.