How to identify Transcription start sites (TSS)
How to identify Transcriptional Start Site (TSS) from Mycobacterium RNA-Seq data?
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Whoknows
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vimlakany •
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vimlakany •
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You could use Homer software for finding TSS regions.
I have copied below command from Homer , read Custom Annotations section precisely: First, you need to convert your bam/sam to bed (bedops works nice) and download GTF file from UCSC website, then run this command:
annotatePeaks.pl ERpeaks.bed hg18 -gtf gencode.gtf > outputfile.txt
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You can not identify the TSS from RNA-Seq. You need CAGE-Seq or GRO-Seq for that.