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Use MEGAN6 to classify functional genes

Dear All,

I used MEGAN6 to analyze my PE reads from Illumina. After blastx reads with nr database (outfmt 0), the results was imported into MEGAN6. And I do click "taxonomy", "SEED", and "KEGG" analysis. However, the taxonomy tree showed well, but all the reads were assigned as "no hits" under both seed and kegg wingdows. I also tried to load the gi mappling file during the importing step, but the problems did not change. Can anyone help to figure it out?

Thanks!

next-gen
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updated by Biostar Biostar • written by wangli_lily • wangli_lily

Can MEGAN import blastx -outfmt 0? I think you have to use -outfmt 6, or xml.

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written by h.mon h.mon

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