Hi

I am a little confused with the megan output. I was wondering if you could please clarify what is the assigned and unassigned reads and how they are calculated. Sorry if I missed anything obvious.

Thanks.

Processing lane5-s001-indexN701-S517-TAAGGCGA-GCGTAAGA-1560_S1_L005_R1_001.fastq.diamond.tsv
Processing BlastTAB file(s)
Note: Reads file(s) not given or found, RMA file will not contain read sequences.
Total reads:   6981911
Total no-hits: 0
Total matches: 121058738
Matches discarded: 959037
Parsing required 2744 seconds
Running Data analyzer: Init
Loading SEED tree files
Loading seed.map: 8324
Loading seed.tre: 9801
Loading KEGG tree files
Loading kegg.map: 9567
Loading kegg.tre: 19923
Loading rn.list: 8270
Analyzing all matches
Number of reads: 6981911
Low complexity:  0
With valid hits: 6981911
With SEED-ids:   404642
With KEGG-ids:   2020891
Writing classification tables
Number of taxa identified: 7187
Number of SEED classes identified:  2521
Number of KEGG classes identified:  3908
Syncing
Data processor required: 1177 secs
Total reads:        6981911
Assigned reads:     6853215
Unassigned reads:   128696
Reads with no hits: 0
Reads low comp.:    0