What does average signal enrichment of a region in Chip-seq peak file means?

Score denotes average signal enrichment for the region. This is written in chip-seq peak files bed format in ENCODE. Can someone explain to me what this means?

chip-seq bed

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updated 3.4 years ago by Ram

• written 10.0 years ago by atsalaki •

1 answer

This is the average (between replicates) read count over the region. If the score is high, it means that a lot of chromatin from that region is pulled down by the IP and sequenced.

Of course, the full analytical method is more complicated than that with filtering, normalization, peak calling, ... If you need more details I suggest you read the ENCODE paper such as this one.

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written 10.0 years ago by Carlo Yague

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