how to convert the MAS5.0 intensity values to normalized log2 value of microarray data. · Biostar

This is a test version of Biostars. For the public version, visit https://www.biostars.org.

how to convert the MAS5.0 intensity values to normalized log2 value of microarray data.

HI every one, I am working on NCBI GEO data for identifying cumulative gene for stress. in the process i have came across the signal intensity values generated from MAS5.0 and GCOS. i am interested to know that is it appropriate to change the intensity values to normalized log2 value. if yes, how to covert the MAS5.0 values to log2. The below given values are example for signal values generated from MAS5.0.

ID_REF VALUE ABS_CALL AFFX-BioB-5_at 363.244 P AFFX-BioB-M_at 311.905 P AFFX-BioB-3_at 399.004 P AFFX-BioC-5_at 259.603 P

sequencing microarray gene assembly blast

• 3,262 views • link

updated 10.0 years ago by Pierre Lindenbaum

• written 10.0 years ago by dinesh •

0 answers

No answers yet.

Log in to answer this question.

More posts like this

Tutorial: Unsupervised clustering on gene expression data
written 4.8 years ago by Hamid Ghaedi

Clustering is a data mining method to identify unknown possible groups of items solely based on intrinsic features and no external variables. Basically, clustering includes …
Using limma for Differential Expression test
written 9.6 years ago by Emilio Marmol

Hello everyone, I'm used to DE analysis using RNA-seq data, but now I have to deal with microarray data. I have this tabultated data: Probe …
Microarray control probes
written 9.9 years ago by mforde84

I'm working with the LINCS 1000 perturbation microarray expression data (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70138). Essentially, it's a bunch of cell lines with expression profiles under a variety of …
Methylation analysis using Gene Spring 12x
written 10.8 years ago by aisharya15 •

<p>Hello everyone,</p> <p>I am using GeneSpring software to analyse methylation data. I've downloaded the data from GEO in text format since its a Illumina file …
Tutorial: Machine Learning For Prediction of Relapse in Cancer - Part 2 - Building A Random Forest …
written 12.7 years ago by Nicholas Spies

This tutorial is part of a series illustrating basic concepts and techniques for machine learning in R. We will try to build a classifier of …