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How to filter vcf file on minimum genotype depth and quality for each sample

How can I filter a vcf filter a VCF file on minimum genotype depth and genotype quality for each sample.

I am looking for a way to filter variants from a VCF file by checking that all samples for a site pass 2 critera

sample.DP > 10
sample.GQ > 15

I thought I could do this using bcftools

bcftools view  -i  'MIN(FMT/DP>10) && MIN(FMT/GQ>15)'   my.vcf.gz

Somehow this include expression does not seem to be applied.

In my output are still lots of variants with genotypes likes 

GT:AO:DP:GQ:PL:QA:QR:RO
0|0:0:16:3:0,48,502:0:554:16   (=depth 16, quality 3)
1|1:1:1:3:21,3,0:37:0:0        (=depth 1, quality 3)

I tried putting the include commands in two different bcftools commands

bcftools view  -i  'MIN(FMT/DP>10)' | bcftools view -i 'MIN(FMT/GQ>15)'   my.vcf.gz

And I tried without the FMT prefix for the genotype quality

bcftools view -i  'MIN(FMT/DP>10)  &&  MIN(GQ>15)'  my.vcf.gz

Still I get variants back with genotypes that don't match the criteria.

Am I misunderstanding the bcftools include expression documentation?

https://samtools.github.io/bcftools/bcftools.html#expressions

Or is there another way that I can achieve this filtering step?

vcf bcftools
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updated by ashotmarg2004 • ashotmarg2004 • written by William William

4 answers

The problem was wrong place of the parentheses:

Does not work as expected:

bcftools view  -i  'MIN(FMT/DP>10) & MIN(FMT/GQ>15)'   my.vcf.gz

Works as expected:

bcftools view  -i  'MIN(FMT/DP)>10 & MIN(FMT/GQ)>15'   my.vcf.gz
• 0 views • link
written by William William

Don't forget to pipe the stdout into a new file. bcftools view --include 'MIN(FMT/DP)>=10 & MIN(FMT/GQ)>=15' my.vcf > my_output.vcf

• 0 views • link
written by LayneSadler LayneSadler

I use vcftools for basic vcf filtering which I find to be very fast and simple to use.

An example would be

vcftools --vcf myfile.vcf --out output_prefix --minGQ 15 --minDP 10 --recode --recode-INFO-all

Manual can be found here https://vcftools.github.io/man_latest.html

• 1 views • link
written by artolkamp • artolkamp

Cools seems to do the job. Thanks.

• 1 views • link
written by William William

At closer look it's not exactly what I was looking for. This command sets the genotypes that don't match the criteria to ./. . I am looking to remove the whole variant if not all genotypes pass the criteria. .

• 1 views • link
written by William William

Does anyone know if there is a way to do this (set genotypes that don't match the criteria to ./.) in bcftools? I'd like to set low-quality genotypes to missing in a multi-sample vcf but keep the sites that contain them, and can't seem to work out how. The vcftools solution works fine, but I'm curious to know if it can be done in bcftools too.

• 1 views • link
written by J.R. • J.R.

from the above script, --minGQ and --minDP take the value of 15 and 10 respectively for filtering genotype quality and depth. Is there any criteria for choosing 15 and 10.. Just to know if 15 and 10 are standard values to use for those flags.

• 0 views • link
written by mab658 mab658

Filtering not on the minimum but on the average GQ and sample DP seems to work for me and the result is close enough to what I was looking for. 

bcftools view -i 'AVG(GQ)>20 & AVG(FMT/DP)>10'
• 1 views • link
written by William William

Hello, Excuse me could you please let me know that the FMT stands for what? Thanks, Omid

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written by jaafari.omid jaafari.omid

In case you have a vcfutils.pl in your pipeline, especially in the famous consensus sequence calling one with mpileup, you can use the -d flag to set the minimum coverage. It also should work alone when converting vcf to fq, for a minimum depth coverage of 10 for example:

  • vcfutils.pl vcf2fq -d 10

vcfutils.pl script exists in the bcftools/bin btw

• 0 views • link
written by FatihSarigol FatihSarigol

Thanks for your helpful answer.

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written by jaafari.omid jaafari.omid

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