bedtools on vcf.gz file
Can bedtools work directly on vcf.gz file, or do you have to first convert this into a vcf file?
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Yes.
bedtools will process gzipped BED, GFF, VCF and BEDPE files in the same manner as uncompressed files. Gzipped files are auto-detected thanks to a helpful contribution from Gordon Assaf.
http://bedtools.readthedocs.io/en/latest/content/overview.html
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