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tools for gene assembly and quality check for sanger sequencing

Hi,

Can anyone advise me please, what is the best tool to use to perform gene assembly after performing sanger sequencing? we aim to sequence around 7 genes using sanger sequencing.

and one more question, is there another tool to check the quality of the fragments before assembly ? (like the case of fastqc in NGS).

many thanks

sanger sequencing fastq gene assembly
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updated by ismaeilmohamed304 • ismaeilmohamed304 • written by lait lait

3 answers

http://www.dnabaser.com/

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written by ismaeilmohamed304 • ismaeilmohamed304

do you know about any free tools ?

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written by lait lait

Did you try Phred and Phrap?

http://www.phrap.org/phredphrapconsed.html

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written by Benn Benn

use dna basser it's for free up to 30 days

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written by ismaeilmohamed304 • ismaeilmohamed304

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