TF and Promoter

I have a novel TF binding site related to my study to important gene promoter so i want to confirm that but i don,t have those samples so what kind of molecular techniques i can use?

chip-seq

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updated 10.2 years ago by Shicheng Guo

• written 10.2 years ago by Azhar •

1 answer

Download TF and TFBS ChIP-seq data from UCSC:

http://hgdownload.cse.ucsc.edu/goldenPath/hg19/encodeDCC/wgEncodeRegTfbsClustered/

And then try to find the cell type you want to validate and therefore you can get what you want to validate.

• 0 views • link

written 10.2 years ago by Shicheng Guo

I have chip seq data for a kind of human tissue , what I already visualized my peak file from ucsc encode data so i ask what should i do experimentally to vaildate

• 1 views • link

written 10.1 years ago by Azhar •

clone the peak region into a report system (vector) and check the fluorescence signal.

• 1 views • link

written 10.1 years ago by Shicheng Guo

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