splice junction file for human(hg19)
Where can I get splice junction annotation file for human(hg19), in order to run tophat with --raw-juncs.
Thanks in advance
rna-seq
sequencing
alignment
splice junction
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Govardhan Anande
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In tophat manual, when "you supply your own transcript annotation data", you should use "--raw-juncs." option.
Normaly(i.e. when dont have our OWN transcript annotation data including splice junction interval), GFF or GTF annotation file includes splice junction informatin.