Is there any reason to analyze plus/minus strands separately in bisulfite seq/methylation data?
I have bisulfite sequencing data that consists of read count data. The data is split based on strand orientation (plus & minus). What sort of questions can be answered by analyzing the plus/minus strand separately?
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Antonio R. Franco
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biohack92
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biohack92
2 answers
The only normal reasons are to test for asymmetric methylation and to see if one particular base in a motif is methylated or not (this can alter factor binding).
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Devon Ryan
In plants, it has been described a non symmetrical methylation in domains other than CpG, such as CHH or CHG See this paper
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Antonio R. Franco
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