Clustalw2 Performing Pairwise-Alignment And Not Multiple-Alignment
$res = exec('C:\\clustalw2 -INFILE=seq.fasta -ALIGN -TYPE=Protein -ktuple=1 -window=5 -score=percent -topdiags=5 -pairgap=3 -pwmatrix=gonnet -pwdnamatrix=iub -pwgapopen=10 -pwgapext=0.1 -matrix=gonnet -dnamatrix=iub -gapopen=10 -gapext=0.2 -gapdist=5 -iteration=none -numiter=1 -clustering=NJ -seqnos=on -outorder=aligned -OUTFILE=res.aln',$out);
print_r($out);
This code runs just fine. But the problem i'm facing is that the clustalw2 is performing only pairwise sequence alignment and not multiple sequence alignment eventhough my file has more than 2 sequences. Where m i goin wrong? Can any one please help!
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Can you post please the log as well?
@Andreas: what do u mean by the log?
Sorry all... My mistake... actually the fasta file was not in the working directory!! The code runs jus fine!!
You might want to just delete this question, if it all turned out to be a mistake in the first place. Glad you figured it out, though!
closing, as the error was found.