Dear Biostar Community,

What is the best tool for read generation from given reference sequences or any that you use/used and it works/worked as expected?

The properties of desired tool:

• reads generate of given insert size and read length with uniform coverage
• handle uniform coverage on circle genomes
• handle large genomes without crashing
• generation of Illumina reads
• generation of PacBio reads
• generation of reads without errors
• generation of reads contaminated by technical sequences
• generation of read-through adapters artefacts

I used ART (http://www.niehs.nih.gov/research/resources/software/biostatistics/art/) but it looks like it uses too old model for Illumina.