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Testing sequence data for selection - one species and several populations

Hello!

I have sequence data for a set of immune genes from several individuals from several populations. I would like to test for patterns of selection within each gene.

Is there a way to do this?

I was thinking of running tests in FUBAR and MEME, but I am unsure if I need to include outgroup(s) in these tests?

Is it possible to take an alignment of e.g. 10 sequences (I know this is a low sample size, but it is what I have) for each of three populations for a given gene and run selection tests on this?

Thank you very much for any advice! Hjalte

dna selection fubar meme

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