Hi,

I am about to use DIYABC with SNPs derived from genomic data. I used GATK to make variant calls followed by hard-filters as suggested in their variant discovery pipeline (Only SNP variants were kept). My gVCF file is composed of 5 populations of the same species (pop1 : 9 individuals, pop2: 6 individuals, pop3: 9 individuals, pop4 : 9 individuals, pop5 : 10 individuals) and 1 individual from an outgroup species.

I was wondering if it was possible to use ABC methods with such low number of individuals and if I could use the single outgroup individual in my analyses or not ?

Thanks for your advices,

Maxime Policarpo