UMI for Exome Target Sequencing
Hi I have a question regarding the use of UMI for Target Re-sequencing.
Incorporating and UMI with the use of a PCR / Amplicon base methods is quite clear for me. But I would like to know How is possible to incorporate an UMI with a target pull down such an Exome or smaller bait set.
Thanks!
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WouterDeCoster
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alessandro.pastore •
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alessandro.pastore •
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After (and perhaps before) the pulldown you normally do a PCR amplification, wouldn't it be possible in your assay to incorporate random nucleotides there in the primer(s) used for amplification? Could you be a bit more specific on your library prep?
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I'm currently looking for how to do phasing with targeted sequencing data. I wonder if that's the reason you are looking into adding UMI to an exome analysis. I'll be highly interested in seeing if there are any valid answers to your question.