Hello, everyone,

I have a very basic question, how to analyze whether gene rank distributions are biased in a gene list wherein each gene can appear multiple times.

For an exammple, in the gene list below, genes A-E appear three times, and genes A and E are likely biased upward (leftward) and downward (rightward), while genes B-D are probably statistically not significantly biased.

geneA geneA geneA geneB geneC geneD geneB geneC geneD geneB geneC geneD geneE geneE geneE

I suppose this is a kind of simlar to gene set enrichment analysis (GSEA), but instead of using external gene lists, I would like to find out, solely from this gene list, that genes A and E are biased with respect to their ranks/positions.

Probably, performing Kolmogorov–Smirnov test for each gene should work, but is there any tool or R script etc to do this kind of simple analyses? Thank you for your kind attention and help.