economy of sequencing

Question

[raya.girish]

hi all one very silly question but i need for knowledge. i m not doing any sequencing so this is little bit new to me

if u have genome to sequence for example viral genome which is small and if you go for sequencing data is produced in GBs. For small genome such large data generation will not be costly?

Answer 1

[Devon Ryan]

For a viral genome you'd normally use one of the smaller machines, like a MiSeq. You'd also likely try to pool samples together (assuming you have more than one). That brings down the per-sample costs to within reason and also limits the amount of data to a more reasonable amount.

Nanopore-based machines (e.g., the MinION) might also be a good option for things like viral genomes. The library prep for those can also be simpler (e.g., that's how Ebola was sequenced in the field).