Hi, has anybody experience in methylation analysis of data generated by Roche GS Junior system? I did bisulfit convertion and pyrosequencing with the GS Junior. I use the AVA-Software of Roche so far, which is suboptimal for this kind of questions. My problem is that the GS Junior outputs a sff-file. Is there any software for methylation analysis which deals with this sff-file? Or is there a way to transform my data, so that other software can read it?