The most popular GO annotation tool
Hi all,
I'm busy with annotation of a de novo assembled transcriptome. For GO annotation, at the first I used blast2go software with loading the blastx results of transcriptome assembly, but the mapping step was so slow. I searched on net for alternative tools and found several for this purpose, but none of them are not as well-known as blast2go. Based on your experiments, could you please suggest me the appropriate tool?
Thanks
1 answer
You should find what you want here: A: Softwares for gene annotation
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It's not a popular tool nor the fastest for this purpose but the way I'd do it would be, if dealing with an animal, to assign each sequence to a TreeFam family using treefamscan and transfer GO annotations by orthology.