Hi all,

I did a blastx of a de novo assembled transcriptome containing about 58000 sequence against Uniprot (viridiplantae) with XML output format. While I expected to finish the job at long time, it took just 3-4 days. I access to a computer with 256 GB RAM and used the command to run blastx:

/blastx -query file1.fasta -db uni_database -out out1.xml -evalue 1e-3 -outfmt 5 -show_gis -num_alignments 15 -num_threads 9

I run as a PBS job on the server and there was no reported error. However, I would like to make sure everything is right to go ahead. Please share your experience.