Underdispersion issue in RNA-Seq data

Dear Altruists,

In most of the Rna-seq studies, people consider the data as overdispersed. I was looking for a paper that deals with underdispersion as well, but couldn't find any.

My question is, why people usually do not consider underdispersion in rna-seq data? Why they assume that Rna-seq count data is overdispersed? Is this because biological samples always shows overdispersion in the data? If so, then why?

Any reference would be really helpful.

Thanks in advance.

differential-expression underdispersion rna-seq

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updated 4.0 years ago by Ram

• written 10.5 years ago by Naim.Mahi •

1 answer

I think your last point is the most salient - we just don't observe underdispersion in practice. Start with the first answer on this previous question and see if it provides some clarity on why this is the case:

Why Does Rna-Seq Read Count Fit Poisson Distribution?

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written 10.5 years ago by Chris Miller

Thanks Chris.

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updated 6.6 years ago by Ram

• written 10.5 years ago by Naim.Mahi •

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