Hello everyone! I am currently working on a cut&run experiment using the cutruntools pipeline. I see in the paper that the pipeline exports a metrics …

Hello, I would like to identify CNVs from a very large exome sequencing dataset (tens of thousands) and the CLAMMS software looks very promising (https://github.com/rgcgithub/clamms). …

Does anyone know if reads, that are marked as duplicates by Picard MarkDuplicates.jar, are taken into account when calculating metrics like 'on bait' or 'on …

<p>Hello all, I have a file with hybrid metrics from aligned exome (X20) data(e.g Genome_size, Bait_territory, Target_territory, Bait_design_efficiency, Total_reads, Pf_reads....etc). How do i calculate the …

<p>Hi,</p> <p>I want to calculate some aligment quality metrics from whole exome data (paired-end Illumina). </p> <p>I used the picard tool CollectAlignmentSummaryMetrics which provides me …

<p>Hi everyone, </p> <p>I don't know which column change when we fixmate the reads using PICARD? could you please tell me what is fixmate? (I …

<p>Hello, </p> <p>I would like to know what is in .sai file after alignment with <a href='http://bio-bwa.sourceforge.net/'>BWA</a>. what should be remove before convert it to …

<p>Hello </p> <p>what is the difference between hg18 and hg19?</p> <p>Thanks </p> <p>Sara</p>

<p>Hi everyone, </p> <p>I have a Sam files and I applied different other analysis but i don't know which column add or change when we …

<p>Hello everyone, </p> <p>I want to do the differentially expressed analysis on the miRNA NGS sequence count data from two library ( control, Condition).</p> <p>what …