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KEGG pathway overlay of user gene list

I want to overlay my differential expressed genes to a known pathway say let us say as an example one pathway in humans - @ http://www.genome.jp/kegg-bin/show_pathway?org_name=hsa&mapno=04310&mapscale=1.0&show_description=show

I want to overlay my differentially expressed genes to display values as well as in different color in above pathway.

Then I followed KEGG Mapper where it is mentioned that one can color the list of genes as-

#hsa    CLP/CMP Blast_phase All
613 #ddccff,red     #ddccff,red
25  #ddccff,red     #ddccff,red
1398    #ddccff     #ddccff
1399    #ddccff     #ddccff
23624   #ddccff     #ddccff
867 #ddccff     #ddccff

However when I tried

hsa04310 PKA #ddccff,red

(1st line of the file), It does not change color. Two questions-

  1. Can someone advice me the format of file which can change at least color of genes and can be pasted @ User data mapping (http://www.genome.jp/kegg/tool/color_a_pathway.html)
  2. In case of 1 above it will not save file in SVG format, Is it possible to extract svg file after overlaying DEG either in KEGG or any other pathway tool. IPA does not provide all comprehensive pathway in one fig rather it uses several sub set of genes to give two three figures which are little difficult to handle after overlaping.

Thanks

kegg user-list

1 answer

This is the usual format

7167 red,blue
C00118 pinK

If there is TAB between gene name and colors, try using SPACE instead. And use RGB or plain color names instead of HEX. I guess KEGG does not work with HEX.

Neither Hex nor RGB is working, I have been trying both of these options restart pathway and all other options are not helpful

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