Hi

I have a problem of understand the result of tophat fusion

when I ran tophat-fusion-post and it generated result.html result.txt

1. Why tophat-fusion-post need both map to genome and nt library?
2. How can I get the seq from the filter result, I know that default is 100bp from html result. But there is many reads, when I want to get more than 100bp
3. I can't understand the

The seventh row is distances (distance1:distance2) between a mate pair and the fusion, i.e., distance1 between the left end of a pair and the left side of the fusion and distance2 between the right end of a pair and the right side of the fusion.The seventh row is distances (distance1:distance2) between a mate pair and the fusion, i.e., distance1 between the left end of a pair and the left side of the fusion and distance2 between the right end of a pair and the right side of the fusion.

3 pairs

31:29
89:27
65:821