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input format file for DiscoSNP software run

I am working on use of discoSNP for my species (reference is not available). I have created fof.txt (fof1.txt, fof2.txt). Also these fof1.txt consist of sample1_R1.fastq sample1_R2.fastq, similarly fof2.txt consist of sample2_R1.fastq sample2_R2.fastq. I am just looking for the SNPs detection among both the samples 1 and 2. I just want to know whether my defined input -T fof.txt is correct as per requirement for discoSNP software to run.

snp discosnp
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updated by Ram Ram • written by Omics data mining Omics data mining

During discoSNP run I am getting this error

Traceback (most recent call last):
  File "/cwd/DiscoSNP++-2.2.1-Source-1/tools/VCF_creator.py", line 155, in <module>
    if variant_object.CheckCoupleVariantID()==1:
  File "/cwd/Disco_SNP/DiscoSNP++-2.2.1-Source-1/tools/ClassVCF_creator.py", line 335, in CheckCoupleVariantID
    bitwiseFlag=int(self.upper_path.listSam[1])
IndexError: list index out of range
... Creation of the vcf file : done ...==> discoRes_k_31_c_auto_D_100_P_1_b_0_coherent.vcf

I am not able to understand "list index out of range".

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updated by Ram Ram • written by Omics data mining Omics data mining

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