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how to normalise Sangar ab1 raw trace data?

Hi,

I am searching for any papers/studies for information regarding the normalization techniques thats applied on ab1 raw trace data ?

sangar ab1 sequencing base calling normalisation
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updated by h.mon h.mon • written by crivenster crivenster

1 answer

Look at the Wikipedia page on Phred scores, it cites several papers describing the statistical treatment applied to ab1 files to get Phred quality scores. Ewing et al (1998) describes normalization steps prior to Phred score estimation, is this what you are looking for?

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written by h.mon h.mon

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