Question

how to normalise Sangar ab1 raw trace data?

0

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8.5 years ago

crivenster ▴ 50

Hi,

I am searching for any papers/studies for information regarding the normalization techniques thats applied on ab1 raw trace data ?

Sangar AB1 sequencing Base calling Normalisation • 2.1k views

ADD COMMENT • link updated 8.5 years ago by h.mon 35k • written 8.5 years ago by crivenster ▴ 50

score 0 · Answer 1 · 2015-10-15

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Entering edit mode

8.5 years ago

h.mon 35k

Look at the Wikipedia page on Phred scores, it cites several papers describing the statistical treatment applied to ab1 files to get Phred quality scores. Ewing et al (1998) describes normalization steps prior to Phred score estimation, is this what you are looking for?

ADD COMMENT • link 8.5 years ago by h.mon 35k