Liquid Volume Unaccounted for on Biomek Machines
My small company has pretty recently started using the Biomek FX and NX machines. We have noticed that it "eats" up a bunch of liquid. For example - we used to make 120mL of reagent for 12 ELISA plates when we were manually pipetting. With the FX (with the 96 channel head), we now make 140mL and there is not even close to 20mL of reagent left in the tray. I double checked the math and programming of my methods. We also noticed this problem with the span-8. It's not a big deal to make extra reagent, I'm just curious as to whether or not this is normal and why it happens. Thank you!
Kara
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