Regarding lecture 2 The FASTQ File Format **
Im trying to do quality control for raw data for a genomics class, here is the fastqc report for Read1!
my questions are:
in this case should I do trimming or masking ?
if trimming, should I trim the position 14-15 and 54-55 ? I could understand the logic behind trimming the 3' end but how I can trim in the middle of the read? Im sorry if my question quite basic but Im really suffering in understanding this concept and if u guys kindly provide me with any reference to read I would highly appreciate ur kindness.