Is it normal that my RNA degradation plot shows more degradation at the 3'-end?

Dear comm,

I have made a RNA degradation plot with the function plotAffyRNAdeg from affy package (Bioconductor) and it seems RNA is more degraded at the 3'-end compared with the 5'. What does it mean? Should I trust my results? Should I correct anything?

BTW, all p-values from summaryRNAdeg function are below 0.05.

Thanks in advance

bioconductor affymetrix microarray r

• 2,337 views • link

updated 3.9 years ago by Ram

• written 10.9 years ago by Juan Cordero

Show the figures so others can give their opinion.

But in general, RNA quality towards 3'ends are of lower quality with respect to 5'-ends.

• 0 views • link

written 10.9 years ago by Chirag Nepal

image: drosophila_RNAdeg

I get a plot with an opposite trend to this one: lower values in the x-axis get higher values in the y-axis, and vice versa.

• 0 views • link

updated 3.9 years ago by Ram

• written 10.9 years ago by Juan Cordero

0 answers

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