Hi there,
I am working on a next generation sequencing dataset and can't seem to find a tool on Galaxy despite extensive searching that can handle degenerate primers. I would like to remove them from my dataset as part of the cleanup steps. Does anyone know of a tool that can do this? P.S. I program in Python (but also somewhat in Perl and R) so writing a script is a possibility, but I am running Galaxy to help with sharing data and analyses with collaborators.
Thanks so much!
Meg
1 answer
If anyone finds that they need this tool, it is now available on the Galaxy ToolShed as "Cut degenerate primers from dataset."
Here is the link: https://toolshed.g2.bx.psu.edu/repository?repository_id=25ad6aeb8b2d047f
It uses a simple Python script.
Cheers,
Meg
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You may have more luck at Galaxy biostars: https://biostar.usegalaxy.org/