Differentailly expressed genes of microarray time series data with replicates...
I have micro array data which is RMA normalized. I want to extract differentially expressed genes.
My data is times series and with replicates.below is the format of my data..(0,4,8,12,16,20) is one day expression and (24,28,32,36,40,44) is 2nd day.
Gene_ID LDT0 LDT4 8 12 16 20 24 28 32 36 40 44
LHY 2243.22 481.2 7.19888 4.78697 10.6717 150.279 1904.97 483.612 8.46222 6.00153 10.8894 135.684
MEA 3.49595 4.04554 3.77595 4.06207 4.03678 4.17023 3.08672 4.18224 3.86883 4.13705 3.54243 4.44046
GBF4 47.3822 72.3035 64.8164 58.9292 52.0097 47.802 54.1476 38.7481 54.5051 83.3657 57.8709 51.4214
CRY2 875.509 839.057 1135.12 1050.2 905.306 1027.61 923.662 836.94 1104.79 1045.74 895.141 1075.92
NFYC9 312.643 337.326 340.071 308.826 252.628 259.479 317.697 295.563 333.879 292.991 319.404 279.003
PIF3 78.576 108.083 68.376 92.5676 32.0555 16.3228 29.381 114.997 124.115 75.6262 61.7131 10.8697
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Okay thank you. I got it.