Obtaining RIN scores with Agilent Bioanalyzer's mRNA program
Hello everyone,
I'm checking the RNA quality from a microbiome extraction on the Agilent Bioanalyzer. I ran the mRNA program so that I could see the % of rRNA present in the sample compared to total RNA.
However, the Bioanalyzer program for mRNA does not provide a RIN (RNA Integrity Number) score, unlike the Total RNA program. Is there a way to obtain a RIN score for traces from the mRNA program? The electropherogram should be the same; I just need a way to apply the algorithm for calculating RIN.
Anyone know how to get the Bioanalyzer program to do this quality check?
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May be this post suites better on Seq answers.
I'll give it a shot over there, too. Trying to cast a wide net.
but cross posting is not encouraged. :)