tophat-fusion for gene fusion detection
I am trying to use tophat-fusion for gene fusion detection.
My data (fastq files) are paired-end, and I have fastq files from multiple lanes, and they are compressed. (fastq.gz).
I am wondering (1) if tophat can take fastq files from multiple lanes in one command line as STAR can; (2) can tophat take fastq.gz files?
Because of the size of the data, it's difficult for me to unzip the fastq files, and merge the ones from multiple lanes into one, it will be not efficient.
Thanks for your help!
-Jackie
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Also, the same questions for deFuse. I want to compare the results from multiple tools.
If I remember right gene fusion finder SOAPfuse and FusionCatcher will do that automatically for you!