Hi,

I am using DEXSeq v1.12 on R 3.1.1 to perfom analysis with total 573267 exons for their differential usage between 5 healthy and 8 patient samples (each ~10 GB BAM file from TopHat).

The job is terminated as memory exceeds the limit (~270 GB RAM). Is there any way to handle scalability of DEXSeq.

Using BPPARAM function in DEXSeq, I tried to submit the job on 10 nodes (each with 8 processors and 36 GB RAM), but the job is getting terminated with the following message:

Aborted by PBS Server
Job aborted on PBS Server initialization
Exit_status=-4 resources_used.cput=00:00:00 resources_used.mem=0kb resources_used.vmem=0kb resources_used.walltime=00:11:58 Error_Path=/dev/pts/0 Output_Path=/dev/pts/0

It appears that DEXSeq is not distributing the job across the nodes.

I am having big trouble with doing dxd = testForDEU( dxd, BPPARAM=BPPARAM), which highly memory intensive step of DEXSeq.

Could somebody please guide me if there is any better way of running this program. Any suggestion is highly appreciated.