what is the best way to look up for a certain nucleotide seq in a specific organism?

Question

[nouranatef.khalil]

Hi all,

I want to look up for the nucleotide sequence of a certain enzyme (levansucrase for example) and I want to look it up in a certain organism (ex: enterobacter sp.)...

I'll write the steps of what I do and please correct me if I'm wrong:

1. I log to NCBI and choose Nucleotide database
2. I write the name of organism AND my enzyme ( ex: Enterobacter AND levansucrase)

but I find that the results are not only restricted to the organism I want, also many of the results don't have the coding sequence of the enzyme I specified in my search..

Is there a better way to find the sequences that I want in a specific organism?

Thank you

Answer 1

[Devon Ryan]

Have a look at the advanced search option. You can use something like levansucrase[Keyword] AND enterobacter[Organism], though there are no hits for that since most sequences for Enterobacter are genomic.

[Siva]

This is the best way to search. OP, you can find here, the list of available Entrez search fields and a brief description of each field.