I guess you have a Position weight matrix. There are different tools that allow you to scan your genomes. Some of them are reported in these posts:

• how to get promoter regions from a genome
• transcription factor binding site prediction software/server

There is also RSAT web site that is really great!

Use FIMO from the MEME suite, selecting the yeast database as target sequence. You can find the yeast genome inside Ensembl Genomes, Genbank Fungii Genomes, and UCSC-other.

You can do the alignment from the online interface, or download the suite in local and do it in your local computer.