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Multiplexed 454 Data Analysis

I'm a newbie and I'd like to know how to start doing analysis of multiplexed 454 data. I know its wide question but I really don't know where to start?

next-gen sequencing
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updated by Johnny Brown • Johnny Brown • written by User 3466 • User 3466

Do, or do not. There is no try. :)

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written by Neilfws Neilfws

Your sequencing provider should demultiplex the data for you, assuming you used the standard Roche MID barcode system.

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written by Torst Torst

2 answers

check out the mothur package, it has 454 flow trimming and demultiplexing features that are very handy and usable even if your data has nothing to do with metagenomics

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written by Istvan Albert Istvan Albert

Qiime may be useful to you. Particularly handy is the MacQiime all-in-one install for apple computers.

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written by Johnny Brown • Johnny Brown

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