problems in Diffbind In Creating Diffbind Object In R For Chip-Seq Peaks
Dear All,
I am trying to input my chip-seq data into diffbind creating diffbind object in R for chip-seq.
My peaks bed file looks like this
chr1 7088771 7089192 MACS_peak_1 99.81
chr1 7902447 7902565 MACS_peak_2 97.40
chr1 9748281 9748621 MACS_peak_3 163.27
........
I created a sample csv file named "chiptest.csv" like this
SampleID,Peaks
1,1peaks.bed
2,2peaks.bed
When I use
tbl1=dba(sampleSheet="chiptest.csv")
I keep having error like this,
1 NA raw
Error in if (is.na(peaks)) { : argument is of length zero
Can anyone help me with imporing my own data into diffbind?
I really would like to use diffbind to analyze my own data.
Many thanks
Whillie
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Did you check that your peaks.bed file is tab delimited not space delimited?
Thanks for your answer. I found mistake at bed file where I had only 5 columns, normally there should be 6 or 9 columns.
Thanks a lot!
Best,
Xiaoyue