Infinium/Illumina 450K methylation array data analysis: How to compare the various packages/pipelines?
Hello
I am planning to work on 450K methylation array data. There are various pipelines/packages for data analysis. I don't need to know the best or the standard pipeline for the analysis. However I would appreciate ideas on how to compare various pipelines?
For a sample data I can use minfi, ChAMP, COHCAP, RnBeads and so forth. But how do I compare which one is working best for me?
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2 answers
In general, to see what algorithm is producing better results, you need to either (A) have a dataset with some known differences or (B) perform an independent validation of some of the discordant results produced by the various packages.
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