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why are all the genes on the positive strand after annotation using Basys annotation server?

i annotated my bacterial sequence with basys annotation server the annotation came back with 7500 gene but all the genes appear to be on the positive strand, so i decided to re annotate with RAST this time 4500 genes came back both on positive and negative strands. i wanted to work with the basys file but why are they all on the positive strand, can any one explain to me what is happening?

sequencing
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updated by Ram Ram • written by samuelksm • samuelksm

Perhaps this from the docs: https://www.basys.ca/server1/basys/cgi/howto.pl

The defline must be in the form >identifier:cstart-end name, where: specifies that the coding sequence is on the complementary strand. If the coding sequence is on the direct strand, just omit the c character.

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updated by Ram Ram • written by Istvan Albert Istvan Albert

Thanks Albert, i contacted the Basys annotation server workers and they just replied the server contained a bug, but they had fixed so i just have to re annotate my sequences.

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written by samuelksm • samuelksm

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