Hello, I've done human exome capture (with Truseq) and sequenced with HiSeq. I found that the ts/tv ratio is ~1.6 for the dozen exomes that I sequenced. I am not sure why this is and wonder if this is a bioinformatics issue (i.e. filtering) or the exome capture issue. I used the same mutation calling pipeline for my earlier exomes using Agilent SureSelect and there my ts/tv ratio was ~3 thanks Les